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Tel. 0049 (0) 6131-392-9292
Fax : 0049 (0) 6131-392-9293
To order send us an E-Mail : info@heparanase.de
Galantos HEParanase Assay: |
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Assay accuracyCV values (intra-assay) are in the range of 2-3 % for manually performed assays and < 2 % for automated assays. Assay Features : |
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Fig. 2: Dose-dependent degradation of labeled heparan sulfate (substrate) by heparanase. (10 µl of purified heparanase are equivalent to 0.1 µU or 0.15 ng of recombinant heparanase). |
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Fig. 3: Dose-dependent inhibition of heparanase enzyme activity by heparin, a known inhibitor of heparanase. The calculated IC50 was 4.4 ng / ml. Recommended Concentration Range for Inhibitors: Potent inhibitors: 0.5 - 10 ng / ml (Based on 100 µl / well for 96-well microplates and 20 µl / well for 384 well plates (e.g. for 384 well plates 0.01 - 0.2 ng / well of sample are needed)) |
